Although medical sciences are progressing tremendously, the diseases caused by viruses, fungi, and bacteria are still an important issue for public health. For this reason, continuous research to determine new antimicrobial compounds is needed. This study investigated the in vitro antimicrobial activity and biochemical composition of Sedum hispanicum ethanol extract for the first time in the literature. The in vitro antimicrobial activity of ethanol extract obtained from Sedum hispanicum by disk diffusion method against seventeen bacteria strains and one fungi strain, and biochemical composition of the ethanol extract was determined by GC-MS. The extract showed antimicrobial activity against all bacteria strains at almost all doses (50, 100, and 200 μL) at different rates, except Pseudomonas fluorescence and Candida albicans. Some compounds found in the extract are not matching with the libraries. As a reason for that, this medicinal plant is proposed to contain some unknown molecules, which should be identified. Our results indicate that research of compounds, particularly unknown ones in the ethanol extract and their antimicrobial activities should be examined further.
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