In vitro Antimicrobial, Antioxidant Activity, and Biochemical Composition of Parasitic Plant Cytinus hypocistis from Turkiye

Mustafa Eray Bozyel1* Kerem Canli2,3 Atakan Benek4 Dilay Turu2 Ozcan Simsek5 Ergin Murat Altuner4

1Canakkale Onsekiz Mart University, Faculty of Arts and Sciences, Department of Biology, Canakkale, Turkiye
2Dokuz Eylul University, Faculty of Science, Department of Biology, Buca, Izmir, Turkiye
3Dokuz Eylul University, Fauna and Flora Research and Application Center, Buca, Izmir, Turkiye
4Kastamonu University, Faculty of Science, Department of Biology, Kastamonu, Turkey
5Canakkale Onsekiz Mart University, Yenice Vocational School, Department of Forestry, Canakkale, Turkiye
* Corresponding author: m.eraybozyel@gmail.com

Cytinus hypocistis (L.) L. (Cytinaceae) is a Mediterranean parasitic plant used traditionally. This study aimed to investigate the in vitro antimicrobial, antioxidant activity, and biochemical composition of C. hypocistis ethanol extract (CHEt). The biochemical composition of CHEt was analyzed by GC-MS. The antimicrobial activity was investigated using a disk diffusion test against 39 bacteria (including 11 multidrug-resistant strains) and 2 fungi strains. Thirteen antibiotics in all strains except MDR strains and thirty-three antibiotics in MDR strains were used as positive controls. The antioxidant activity was determined by the DPPH method. The major component is 1,2,3-Benzenetriol. CHEt has antimicrobial activity against sixteen strains. CHEt displayed the highest activity against Enterococcus faecium (FI2) is a Gram-positive bacteria strain in 100 μL with a 23 mm inhibition zone. Also, Proteus vulgaris (MDR6), which is Gram-negative bacteria, is the most sensitive strain with a 24 mm inhibition zone in 100 μL of CHEt. Especially, the data from the A. baumannii (MDR3) strain is very striking because a higher activity has been observed than all positive controls. CHEt showed high antioxidant activity against ascorbic acid. In light of all the results, it was determined that C. hypocistis has high antimicrobial and antioxidant potential.
Keywords:Cytinus hypocistis, GC-MS, disk diffusion, DPPH, Multidrug-resistant bacteria

How to cite: Bozyel ME, Canli K, Benek A, Turu D, Simsek O, Altuner EM. (2023). In vitro Antimicrobial, Antioxidant Activity, and Biochemical Composition of Parasitic Plant Cytinus hypocistis from Turkiye. Fresenius Environmental Bulletin, 32(3): 1266-1273.

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