Biochemical and Antioxidant Properties as well as Antimicrobial and Antibiofilm Activities of Allium scorodoprasum subsp. jajlae (Vved.) Stearn

Kerem Canli^1,2*, Dilay Turu³, Atakan Benek⁴, Mustafa Eray Bozyel¹, Ozcan Simsek⁵, Ergin Murat Altuner⁶

¹Dokuz Eylul University, Faculty of Science, Department of Biology, Izmir, Turkey

²Dokuz Eylul University, Fauna and Flora Research and Application Center, Izmir, Turkey

³Dokuz Eylul University, Graduate School of Natural and Applied Sciences, Department of Biology, Izmir, Turkey

⁴Kastamonu University, Graduate School of Natural and Applied Sciences, Department of Biology, Kastamonu, Turkey

⁵Canakkale Onsekiz Mart University, Yenice Vocational School, Department of Forestry, Canakkale, Turkey

⁶Kastamonu University, Faculty of Science, Department of Biology, Kastamonu, Turkey

* Corresponding author: kerem.canli@deu.edu.tr

In this study, the chemical composition and biological activity of Allium scorodoprasum subsp. jajlae (Vved.) Stearn were investigated for the first time, focusing on its antimicrobial, antioxidant, and antibiofilm properties. A GC-MS analysis was employed to evaluate the composition of its secondary metabolites, identifying linoleic acid, palmitic acid, and octadecanoic acid 2,3-dihydroxypropyl ester as the major compounds in ethanol extract. The antimicrobial activity of A. scorodoprasum subsp. jajlae was assessed against 26 strains, including standard, food isolate, clinical isolate, and multidrug-resistant ones, as well as three Candida species using the disc diffusion method and the determination of the minimum inhibitory concentration (MIC). The extract showed strong antimicrobial activity against Staphylococcus aureus strains, including methicillin-resistant and multidrug-resistant strains, as well as Candida tropicalis and Candida glabrata. Its antioxidant capacity was evaluated using the DPPH method, revealing a high level of antioxidant activity in the plant. Additionally, the antibiofilm activity of A. scorodoprasum subsp. jajlae was determined, demonstrating a reduction in biofilm formation for the Escherichia coli ATCC 25922 strain and an increase in biofilm formation for the other tested strains. The findings suggest potential applications of A. scorodoprasum subsp. jajlae in the development of novel antimicrobial, antioxidant, and antibiofilm agents.

Keywords: Allium scorodoprasum subsp. jajlae; antimicrobial activity; antioxidant activity; antibiofilm activity; GC-MS analysis

How to cite: Canli K, Turu D, Benek A, Bozyel ME, Simsek Ö, Altuner EM. (2023) Biochemical and Antioxidant Properties as well as Antimicrobial and Antibiofilm Activities of Allium scorodoprasum subsp. jajlae (Vved.) Stearn. Current Issues in Molecular Biology, 45(6):4970-4984.

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