Synthesis, Characterization, Antioxidant, Antibacterial and Enzyme Inhibitor Evaluation of New Furan-Tethered 1,3,4-Thiadiazoles

Halit Muglu¹, Mohamed Ab. Khalifa Ibrahim¹, Hasan Yakan^2*, Omer Tas³, Gurkan Bilir³, Ercument Aksakal⁴, Temel Kan Bakir¹, Ergin Murat Altuner⁵, Murat Senturk⁶, Deniz Ekici^3*

¹Kastamonu University, Faculty of Science, Department of Chemistry, Kastamonu, Türkiye

²On Dokuz Mayis University, Department of Chemistry Education, Samsun, Türkiye

³On Dokuz Mayis University, Department of Agricultural Biotechnology, Samsun, Türkiye

⁴Akdeniz University, Department of Agricultural Biotechnology, Antalya, Türkiye

⁵Kastamonu University, Faculty of Science, Department of Biology, Kastamonu, Türkiye

⁶Agri Ibrahim Cecen University, Department of Basic Sciences of Pharmacy, Agri, Türkiye

* Corresponding author: hasany@omu.edu.tr; deniz.ekinci@omu.edu.tr

Novel furan-tethered 1,3,4-thiadiazoles (1-5) were prepared and characterized by FT-IR, 1H NMR, 13C NMR and elemental analysis. Compounds, in general, showed antibacterial effects on selected bacteria, including K. pneumoniae, E. faecium, S. epidermidis, S. macrescens, B. subtilis, E. coli, and S. kentucky. Besides, none of the compounds had effects on P. aeruginosa. In vitro antioxidant activity determination was carried out using the DPPH method, and results of the synthesized compounds were found to be close to each other showing lower antioxidant activity than the standard used (ascorbic acid). In vitro acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibition of the synthesized compounds was performed using Ellman's spectrophotometry method. The compounds showed inhibition with IC₅₀ values ranging from 27.39 to 45.71 µM for AChE and 13.35 to 115.17 µM for BChE. Among the synthesized derivatives, compound 2 exhibited the strongest inhibitory effect against both AChE, and BChE.

Keywords: 1,3,4-thiadiazoles, spectroscopic methods, antibacterial activity, antioxidant activity, AChE and BChE inhibition

How to cite: Muglu H, Khalifa Ibrahim MA, Yakan H, Tas O, Bilir G, Aksakal E, Bakir TK, Altuner EM, Senturk M, Ekici D. (2026) Synthesis, Characterization, Antioxidant, Antibacterial and Enzyme Inhibitor Evaluation of New Furan-Tethered 1,3,4-Thiadiazoles. Revue Roumaine de Chimie, 71(3-4), 155-168.

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