The Fomes fomentarius methanol extract and antibiotic interactions in some clinically isolated multidrug resistant E. coli strains with active efflux pumps by using FTIR analysis
Studies to prevent antibiotic resistance are continuing rapidly. As it is known, bacteria have various resistance mechanisms. The over-expression of efflux pumps is just one of these resistance mechanisms. In antibiotic intake, the drug taken through this resistance mechanism is expelled out of the cell and the effect of the drug is lost. Various studies are carried out to prevent this. Inhibition of the efflux pumps is one of these methods. With the inhibitory effect of compounds, it is aimed to recover the effect of drugs in the in vitro studies. In order to avoid the toxicity problem, the researches for inhibitors from natural sources are under investigation. In this study, methanol extract of Fomes fomentarius macrofungus was used to observe its efflux pump inhibition potential in some clinically isolated multidrugresistant Escherichia coli strains. Antibiotics were selected according to the antibiotic resistance profile of the strains used and the study was carried out using a combination with the extract. FTIR analysis was performed to evaluate the mechanism of action for strains with an inhibitory effect as a result of combined use. According to the FTIR analysis; there are significant changes in the band scale. The observed changes were found in proteins, DNA/RNA, phospholipids, polysaccharides, fatty acids, and fingerprint regions. In light of the observed data, it is appropriate to elaborate further studies.
Altınoz, E., Altuner, E.M. (2022). Observing the presence of efflux pump activities in some clinically isolated bacterial strains. International Journal of Biology and Chemistry, 15(1): 48-54.
Altınoz, E., Altuner, E.M. (2020). Responses of some Escherichia coli clinical isolate strains with multiple drug resistance and overexpressed efflux pumps against efflux pump inhibitors. International Journal of Biology and Chemistry, 13(1): 77-87.
Altınoz, E. (2019). Çoklu İlaç Direnci Gösteren Ve Efflux Pompa Sistemi Çalışan Escherichia Coli Suşlarının Efflux Pompası İnhibitörlerine Karşı Cevaplarının Gözlenmesi. (Yüksek Lisans Tezi, Kastamonu Üniversitesi).
Altınoz, E., Altuner, E.M. (2019). Antibiotic resistance and efflux pumps. International Journal of Innovative Research and Reviews, 3(2): 1-9.