The FTIR analysis of Fomes fomentarius ethyl acetate extract-antibiotic interactions in some multi-drug resistant (MDR) E. coli strains having active efflux pumps
Studies on eliminating multi-antibiotic resistance are continuing and in these studies, the search for inhibitors takes a major role. Due to the toxic nature of synthetic efflux pump inhibitors, new inhibitors having low toxicity are under investigation. Although studies at the in vivo level are long-term, studies at the in vitro level are provided more easily. In this study, inhibitor trials of some multidrug-resistant Escherichia coli strains with active efflux pumps were carried out using ethyl acetate extract of Fomes fomentarius, a macrofungi. In addition, antibiotics were selected according to the antibiotic resistance profile of the strains used, and combined use with the extract was tried. According to the results of the combined use of antibiotics and extracts, FTIR analysis was performed for microorganisms presenting an inhibitory effect. Thus, preliminary studies will be carried out by evaluating the mechanism of action. According to the FTIR results; significant changes were observed between the band <900 cm-1 and 3000 cm-1. While some of these changes occurred in proteins, some changes occurred in DNA/RNA and phospholipids. The obtained data will be detailed and used for further studies.
Altinoz, E., Altuner, E.M. (2022). Observing the presence of efflux pump activities in some clinically isolated bacterial strains. International Journal of Biology and Chemistry, 15(1): 48-54.
Altinoz, E., Altuner, E.M. (2020). Responses of some Escherichia coli clinical isolate strains with multiple drug resistance and overexpressed efflux pumps against efflux pump inhibitors. International Journal of Biology and Chemistry, 13(1): 77-87.
Altinoz, E. (2019). Çoklu İlaç Direnci Gösteren Ve Efflux Pompa Sistemi Çalışan Escherichia Coli Suşlarının Efflux Pompası İnhibitörlerine Karşı Cevaplarının Gözlenmesi. (Yüksek Lisans Tezi, Kastamonu Üniversitesi).
Altinoz, E., Altuner, E.M. (2019). Antibiotic resistance and efflux pumps. International Journal of Innovative Research and Reviews, 3(2): 1-9.