Antibiotic resistance has become the biggest problem of the 21st century. It is estimated that this problem will result in around 10 million deaths per year by 2050. Macrofungi are very important for the discovery of new antibiotics as natural sources with potential microbial effects. In this study, the effect of the ethanol extract of P. hartigii (PH-EtOH), which was known to have antimicrobial properties against various microorganisms, was evaluated on Trimethoprim/Sulfamethoxazole (SXT) resistant Acinetobacter lwoffii with MIC, MBC tests and analyzed with FTIR to understand the changes at the molecular level. It has been determined by MIC and MBC tests that PH-EtOH has an antibacterial effect against A. lwoffii. FTIR spectra of PH-EtOH treated and untreated samples were analyzed and it was determined that significant differences occurred in lipid (3000-2800 cm-1), protein (1800-1200 cm-1) and nucleic acid (1200-900 cm-1) specific spectral ranges. The spectral differences in these regions allowed a comprehensive understanding of the effects of PH-EtOH extract at the molecular level, such as changes in protein secondary structures and functional groups of A. lwoffii, changes in lipid composition and membrane structure, and structural and functional modifications in nucleic acid components. In addition, Amide I/Amide II, RNA/DNA, Amide I/RNA and Amide I/DNA ratios were calculated to examine changes in the structures and compositions of these molecules.
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